| Description: | The DX-236(FWFCDRIAWYPQHLCEFL)-Sepharose column was the best performer and quantitatively bound the entire 1 mg injection (0.15 μmol). At higher HSA loads in the no salt, pH 6.2 buffer, the DX-236-Sepharose column (0.35 mL) bound upward of 4 mg of human serum albumin (HSA), which corresponds to a dynamic capacity of greater than 11 mg/mL. The DX-236 affinity column bound HSA from human serum with a greater specificity than does Cibacron Blue agarose beads. DX-236 also bound well to several mammalian serum albumins. |
