BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1815

Peptides:

Sequences

RSNTRMTARQHRSANHKSTQRARS(8)
RSVFLPSILGWRSRLDDQGVAARS(2)
RSTRNKHTTARRSVAPGIGEPSRS(3)
RSIMHVRLRARRSARHMKDADPRS(1)
RSPIIIRSRINRSHGRTKATPARS(1)
RSRGLRNILMLRSYDSRSMRPHRS(2)
RSTRRGTHNKDRS(4)
RSTVPKRHPKDRS(1)
RSIAKKTHNKQRS(1)
RSYDSRSMRPHRS(1)
RSTASRHTEPHRS(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Bacterial display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 10618196

Target Name:

Zinc oxide, ZnO

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X9 E. coli bacterial display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

ZnO and bacteria expressing the random peptide library in FimH were mixed and allowed to adhere to each other at room temperature with gentle agitation. Centrifugation was then performed, and the ZnO and any adhering bacteria were recovered and inoculated into Luria-Bertani medium containing the appropriate antibiotics. After overnight incubation, exponentially growing cultures were established, and the enrichment procedure was repeated.

BiopanningDataSet Comments:

The sequences selected exhibited various degrees of affinity and specificity towards ZnO. Competitive binding experiments revealed that the sequences recognized only the oxide form of Zn. Interestingly, one of the inserts, RSNTRMTARQHRSANHKSTQRARS, exhibited significant homology to a specific sequence in a putative zinc-containing helicase, which suggests that searches such as this one may aid in identifying binding motifs in nature.

Curator:

Curation Date:

0000-00-00

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