BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1758

Peptides:

Sequences
CETGAKPHC(0.75) CQTGEKPQC(0.083) CETGEKPQC(0.083) CQAFFPNAC(0.083)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 16087122

Target Name:

Large envelope protein

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Saturation equilibrium assay

Affinity Measurement Description:

Results of equilibrium binding assay show that the concentration of the free phage clone CETGAKPHC reduces progressively with increased concentration of HBsAg. The binding data were fitted with the equation for two binding sites and the Krel D values were 2.9±0.9 nM (KrelD1) and 0.83±0.63 mM (KrelD2).

Brief Description:

BiopanningDataSet Comments:

The binding site of peptide ETGAKPH was located on the immunodominant region of HBsAg. An equilibrium binding assay in solution showed that the phage binds tightly to HBsAg with a relative dissociation constant (KDrel) of 2.9+/-0.9 nM. The phage bearing this peptide has the potential to be used as a diagnostic reagent and two assays for detecting HBsAg in blood samples are described.

Curator:

Curation Date:

0000-00-00

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