| General information | |
| ACovPid: | ACoVP100408 |
| Trivial Name: | N3HR |
| Amino Acids Sequence: | (IEEIVEEIHHIERAIQQIEQRIQQIEQRIQQIEQRIL)3 |
| Length: | 37 |
| C-Terminal Modification: | None |
| N-Terminal Modification: | None |
| Chemical Modification: | None |
| Peptide Source: | Human immunodeficiency virus -1 gp41 : 33929200 |
| Source Description: | We engineered the mimetic of the gp41 HR1 helical trimer, designated N3G, on the basis of the N37 sequence and the use of combined strategies, including isoleucine substitution at the a–d sites in the second heptad, intrahelical salt-bridge introduction i |
| Against Virus: | |
| Inhibition Value Type: | IC50 |
| Inhibitory Effect: | >20 |
| Inhibitory Unit: | µM |
| Target Domain Name: | |
| Assay: | Cell–cell fusion assay |
| Assay Description: | The target cells were Huh-7 cells expressing the MERS-CoV receptor dipeptidyl peptidase 4. The effector cells were 293T/MERS/enhanced GFP protein (EGFP) cells. The 293T/MERS/EGFP cells were transfected with a plasmid containing the MERS-CoV S protein gene and the EGFP gene. The 293T/EGFP cells expressing only EGFP were employed as negative control cells. Huh-7 cells were plated in 96-well plates (5 × 10^4 cells/well) at 37 °C for 5 h. Then, 293T/MERS/EGFP cells or 293T/EGFP cells (1 × 10^4 cells/well) with or without serially diluted peptide were added. After being co-cultured at 37 °C for 2 h, the fused and unfused cells were counted under an inverted fluorescence microscope (Nikon Eclipse Ti-S). |
| Anti-CoV activity in vivo: | |
| Reference: | 33929200 |
| Comment: | |
| 3D structure: | |
| Structure Experiment Verified: | |
| Similar Peptides: | |