| General information | |
| ACovPid: | ACoVP100402 |
| Trivial Name: | Peptide #3 |
| Amino Acids Sequence: | DISGINASVVNIQKEIDRLNEVAKNLNESLIELQEL |
| Length: | 36 |
| C-Terminal Modification: | None |
| N-Terminal Modification: | None |
| Chemical Modification: | None |
| Peptide Source: | Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) / Severe acute respiratory syndrome (SARS) coronavirus 2 (SARS-CoV-2) / Middle East respiratory syndrome coronavirus (MERS-CoV) : 694009/2697049 |
| Source Description: | From the heptad repeat region 2 of spike protein |
| Against Virus: | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) : 2697049 |
| Inhibition Value Type: | IC50 |
| Inhibitory Effect: | 7.2 |
| Inhibitory Unit: | µM |
| Target Domain Name: | |
| Assay: | DSP assay DSP assay for SARS-CoV-2 S-mediated cell-cell fusion |
| Assay Description: | The DSP assay using 293FT cells was performed as described previously (PMID: 32532094) to monitor SARS-CoV-2-S-mediated membrane fusion. Briefly, effector cells expressing SARS-CoV-2-S protein with DSP8-11, target cells expressing ACE2, and transmembrane serine protease 2 (TMPRSS2) with DSP1-7 were seeded in 10 cm culture dishes (4×10^6 cells/10 mL) one day before the assay. Two hours before the DSP assay, cells were treated with 6 µM EnduRen (Promega, Madison, WI, USA), a substrate for Renilla luciferase, to activate EnduRen. One microliter of each peptide dissolved in dimethyl sulfoxide (DMSO) was added to the 384-well plates (Greiner Bioscience, Frickenhausen, Germany). Next, 50 µL of each single-cell suspension (effector and target cells) was added to the wells using a Multidrop dispenser (Thermo Fisher Scientific, Waltham, MA, USA). After incubation at 37°C for 4 h, luciferase activity was measured using a Centro xS960 luminometer (Berthold, Bad-Wildbad, Germany). |
| Anti-CoV activity in vivo: | |
| Reference: | 33424013 |
| Comment: | The concluded sequence for peptide synthesis was generated after alignment of SARS-CoV, SARS-CoV-2, and MERS-CoV HR2 sequences. |
| 3D structure: | |
| Structure Experiment Verified: | NO |
| Similar Peptides: | ACoVP100125 ACoVP100126 ACoVP100119 ACoVP100118 ACoVP100120 |