| General information | |
| ACovPid: | ACoVP100393 |
| Trivial Name: | M-wt |
| Amino Acids Sequence: | KYEQYIKWPWYVWLGF |
| Length: | 16 |
| C-Terminal Modification: | None |
| N-Terminal Modification: | None |
| Chemical Modification: | None |
| Peptide Source: | Severe acute respiratory syndrome coronavirus (SARS-CoV) : 694009 |
| Source Description: | |
| Against Virus: | Severe acute respiratory syndrome coronavirus (SARS-CoV) : 694009 |
| Inhibition Value Type: | IC50 |
| Inhibitory Effect: | 26 |
| Inhibitory Unit: | µM |
| Target Domain Name: | |
| Assay: | in Vitro assay |
| Assay Description: | The ability of MPER in both complementary interaction (with IFP) and self-interaction raised the possibility that MPER-derived peptides could serve as viral entry inhibitors. In principle, both MPER and IFP are exposed during viral entry, and their association with a MPERderived peptide could inhibit the progression of viral entry and infection. For safety reasons, the inhibitory activity of M-wt peptide was tested in neutralization assays using avian infectious bronchitis virus (IBV), instead of SARS-CoV. IBV contains the same MPER sequence and undergoes a fusion process synonymous to that of SARS-CoV. Specifically, the assay was conducted on recombinant IBV-Luc, which contains a firefly luciferase gene integrated at its ORF3a3b. Successful viral entry and subsequent replication of IBV-Luc were assessed by intracellular luciferase activity. The MPER peptide derived from IBV, M-ibv (LKTYIKWPWYVWLAIAF), was tested in parallel. Increasing concentrations of synthetic MPER peptides (0, 12, 25, and 50 µM) were mixed with IBV-Luc for 1 h at 37 °C and applied to Vero E6 cells seeded in a 96-well plate at 1 PFU. The peptide/virus mixture was removed 1 h postinfection. Cells were lysed 20 h postinfection, and luciferase activity was measured. |
| Anti-CoV activity in vivo: | |
| Reference: | 25668103 |
| Comment: | |
| 3D structure: | |
| Structure Experiment Verified: | NO |
| Similar Peptides: | ACoVP100218 ACoVP100228 ACoVP100242 ACoVP100246 ACoVP100250 |