General information
    ACovPid:ACoVP100390
    Trivial Name:SR9
    Amino Acids Sequence:ISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELG
    Length:35
    C-Terminal Modification:None
    N-Terminal Modification:None
    Chemical Modification:None
    Peptide Source:

    Severe acute respiratory syndrome coronavirus (SARS-CoV) : 694009

    Source Description:From the heptad repeat region 2 of spike protein of SARS-CoV
    Against Virus:

    Severe acute respiratory syndrome coronavirus (SARS-CoV) : 694009

    Inhibition Value Type:EC50
    Inhibitory Effect:0.005
    Inhibitory Unit:µM
    Target Domain Name:HR1 domain of SARS-CoV
    Assay:in Vitro assay
    Assay Description:To estimate these sHRPs, we established a rapid and virus-free in vitro novel assay system based on the inhibition of HR1-HR2 complex formation. Two fusion proteins (maltose binding protein [MBP]-HR1 [amino acid residues of the S protein, 892 to 964] and glutathione S-transferase [GST]-HR2 [1141 to 1192]) were expressed using Escherichia coli and purified using amylose resin (New England Biolabs) and glutathione Sepharose 4B (GE Healthcare, Bucks, United Kingdom), respectively. An enzyme-linked immunosorbent assay plate was coated with GST-HR2 dissolved in sodium carbonate buffer (pH 8.5), 3.6 µg/ml in concentration, by incubation at 4°C for 8 h. After bovine serum albumin blocking (1 mg/ml) at 4°C for 2.5 h, GST-HR2 on the plate was allowed to bind the MBP-HR1 protein (8.8 µg/ml) by incubation at 37°C for 1.5 h in the presence of various concentrations of sHRPs to be examined for inhibition activity. After the plate was washed, the inhibiting potency of the peptide was assessed by colorimetric analyses using the anti-MBP antibody-alkaline phosphatase conjugate (Sigma) with a 1:1,000 dilution with incubation at 4°C for 1 h and then staining with BluePhos microwell phosphatase (KPL).
    Anti-CoV activity in vivo:
    Reference:17942557
    Comment:
    3D structure:

    StructureACoVP100390

    Structure Experiment Verified:NO
    Similar Peptides:ACoVP100242   ACoVP100125   ACoVP100119   ACoVP100120   ACoVP100118

    Target Domain information
    Target Domain Full Name:Heptad repeat 1 (HR1) domain of Severe acute respiratory syndrome coronavirus (SARS-CoV) spike glycoprotein
    Target Type:glycoprotein
    UniprotID [Sequence]:

    P59594 [902-952]

    Target Synonyms:Alternative name(s) for spike glycoprotein: E2 Peplomer protein S glycoprotein
    Target Source:

    Severe acute respiratory syndrome coronavirus (SARS-CoV) : 694009

    Target Structure:1WNC, 1WYY, 1ZV7, 1ZV8, 1ZVB, 2AJF, 2BEQ, 2BEZ, 2DD8, 2FXP, 2GHV, 2GHW, 2RUM, 2RUN, 2RUO, 3BGF, 3D0G, 3D0H, 3D0I, 3SCI, 3SCJ, 3SCK, 3SCL, 5WRG, 5X4S, 5X58, 5X5B, 5XJK, 5XLR, 5ZVM, 6ACC, 6ACD, 6ACG, 6ACJ, 6ACK, 6CRV, 6CRW, 6CRX, 6CRZ, 6CS0, 6CS1, 6CS2, 6M3W, 6NB6, 6NB7, 6VW1, 6WAQ, 7JN5