General information
    ACovPid:ACoVP100370
    Trivial Name:K10
    Amino Acids Sequence:DLKGKYVQIP
    Length:10
    C-Terminal Modification:None
    N-Terminal Modification:None
    Chemical Modification:None
    Peptide Source:

    Severe acute respiratory syndrome coronavirus (SARS-CoV) : 694009

    Source Description:Sequence of nsp 10 of SARS-CoV
    Against Virus:

    Severe acute respiratory syndrome coronavirus (SARS-CoV) : 694009

    Inhibition Value Type:IC50
    Inhibitory Effect:
    Inhibitory Unit:
    Target Domain Name:
    Assay:SAM binding assays
    Assay Description:25 µl reaction mixtures [40 mM Tris–HCl (pH 7.5), 2 mM MgCl2, 2 mM DTT] containing 0.5 µM purified nsp16, different concentrations of nsp10 or its truncations and 2 µCi of S-adenosyl [methyl-3H] methionine (67.3 Ci/mmol, 0.5 µCi/µl) were pipetted into wells of a microtiter plate. The reaction mixtures were incubated on ice and irradiated with 254-nm UV light in a Hoefer UVC500 cross-linking oven for 30 min. The distance of samples from the UV tubes was 4 cm. The samples were then analyzed by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The gels were socked in Enlightening buffer (PerkinElmer) and analyzed by autoradiography
    Anti-CoV activity in vivo:
    Reference:22659295
    Comment:
    3D structure:

    StructureACoVP100370

    Structure Experiment Verified:NO
    Similar Peptides:ACoVP100373