| General information |
| ACovPid: | ACoVP100324 |
| Trivial Name: | IIQ |
| Amino Acids Sequence: | IEEIQKKIEEIQKKIEEIQKKIEEIQKKIEEIQKK-βA-K(C16) |
| Length: | |
| C-Terminal Modification: | None |
| N-Terminal Modification: | None |
| Chemical Modification: | None |
| Peptide Source: |
| Source Description: | Initially, the author aimed to establish combinations of foregrounda-d residues in the heptad repeat moiety. As previous experiments have indicated that alanine residues are strongly biased toward helix formation in peptides,38 the design of the novel lip |
| Against Virus: | The Middle East respiratory syndrome coronavirus (MERS-CoV) |
| Inhibition Value Type: | EC50 |
| Inhibitory Effect: | 0.13 |
| Inhibitory Unit: | µM |
| Target Domain Name: | |
| Assay: | Pseudotyped virus infection inhibition assay |
| Assay Description: | Inhibition of MERS-CoV pseudovirus infection was assessed using a previously described method(PMID: 26164863). Briefly, the serially diluted tested peptides were added to a 96-well plate and incubated with MERS-CoV pseudovirus for 30 min at 37 °C. Then, the pseudovirus/peptide mixture was added to the Huh-7 cells. Cultures were refed with fresh medium at 12 h postinfection and incubated for an additional 48 h at 37 °C. Fluorescence was determined using a luciferase kit (Promega) and an Ultra 384 luminometer (Tecan). |
| Anti-CoV activity in vivo: | |
| Reference: | 30192544 |
| Comment: | |
| 3D structure: |
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| Structure Experiment Verified: | |
| Similar Peptides: | |
