| General information |
| ACovPid: | ACoVP100321 |
| Trivial Name: | IIK |
| Amino Acids Sequence: | IEEIKKKIEEIKKKIEEIKKKIEEIKKKIEEIKKK-βA-K(C16) |
| Length: | |
| C-Terminal Modification: | (C16): palmitoyl group; |
| N-Terminal Modification: | None |
| Chemical Modification: | βA: β-alanine; |
| Peptide Source: |
| Source Description: | Initially, the author aimed to establish combinations of foregrounda-d residues in the heptad repeat moiety. As previous experiments have indicated that alanine residues are strongly biased toward helix formation in peptides,38 the design of the novel lip |
| Against Virus: | The Middle East respiratory syndrome coronavirus (MERS-CoV) |
| Inhibition Value Type: | EC50 |
| Inhibitory Effect: | 0.45 ± 0.13 |
| Inhibitory Unit: | µM |
| Target Domain Name: | |
| Assay: | Cell–cell fusion assay |
| Assay Description: | The target cells were Huh-7 cells expressing the MERS-CoV receptor dipeptidyl peptidase 4. The effector cells were 293T/MERS/enhanced GFP protein (EGFP) cells. The 293T/MERS/EGPF cells contained the MERS-CoV S protein gene and the EGFP gene transfected with plasmid. The 293T/EGFP cells expressing only EGFP were employed as negative control cells. Huh-7 cells were plated in 96-well plates (5 × 10^4 cells/well) at 37 °C for 5 h. Then, serially diluted peptide samples were added, followed by the addition of 293T/MERS/EGPF cells or 293T/EGFP cells (1 × 10^4 cells/well). After coculturing at 37 °C for 4 h, the 293T/MERS/EGFP cells and 293T/EGFP cells, either fused or unfused, with Huh-7 cells were counted under an inverted fluorescence microscope (Nikon Eclipse Ti-S). |
| Anti-CoV activity in vivo: | |
| Reference: | 30192544 |
| Comment: | |
| 3D structure: |
|
| Structure Experiment Verified: | |
| Similar Peptides: | |
