| General information | |
| ACovPid: | ACoVP100306 |
| Trivial Name: | P21S8Z |
| Amino Acids Sequence: | LDLTYE-LN-LSLQVVKLNESY |
| Length: | 19 |
| C-Terminal Modification: | None |
| N-Terminal Modification: | None |
| Chemical Modification: | LN: L-norleucine; |
| Peptide Source: | The Middle East respiratory syndrome coronavirus (MERS-CoV) : |
| Source Description: | S2 subunit of spike (S) protein of MERS-CoV |
| Against Virus: | |
| Inhibition Value Type: | EC50 |
| Inhibitory Effect: | 4.15 ± 0.25 |
| Inhibitory Unit: | µM |
| Target Domain Name: | |
| Assay: | Pseudotyped virus infection inhibition assay |
| Assay Description: | MERS-CoV pseudovirus was generated via cotransfection of 293T cells with the plasmid pNL4-3.luc.RE as well as the pcDNA3.1-MERS-CoV-S plasmid, as previously described(PMID: 23978242). Briefly, 293T cells (ATCC, Manassas, VA) were co-transfected with 20 µg of plasmid encoding Env-defective, luciferase-expressing HIV-1 (pNL4-3.luc.RE) and 20 µg of rMERS-CoV-S plasmid, respectively, into a-T175 tissue culture flask using the calcium phosphate method. Cells were changed into fresh DMEM 8 h later. Supernatants were harvested 72 h post-transfection and used for single-cycle infection. The plasmids encoding vesicular stomatitis virus G protein (VSV-G-pcDNA3.1) and pcDNA3.1 vector were co-transfected with pNL4-3.luc.RE plasmid to generate VSV-G pseudovirus and pseudovirus without Env (Env-) as controls. Peptides at graded concentrations were mixed with the pseudovirus and then incubated for 1 h at room temperature. The mixture was added to Huh-7 or Calu-3 cells, fresh medium was added 12 h later, and the cells were incubated for an additional 48 h at 37 °C. Fluorescence was determined immediately using a luciferase kit (Promega) and an Ultra 384 luminometer (Tecan) after the addition of luciferase substrate (Promega). |
| Anti-CoV activity in vivo: | |
| Reference: | 29442512 |
| Comment: | For Q1020H-MERS-CoV pseudovirus |
| 3D structure: | |
| Structure Experiment Verified: | |
| Similar Peptides: | ACoVP100296 ACoVP100290 ACoVP100284 ACoVP100289 ACoVP100281 |