General information
    ACovPid:ACoVP100293
    Trivial Name:P21R8
    Amino Acids Sequence:LDLTYEMLSLQ-RPA-VVK-RPA-LNESY
    Length:
    C-Terminal Modification:None
    N-Terminal Modification:None
    Chemical Modification:RPA: (R)-2-(4-pentenyl) alanine;  
    Peptide Source:

    The Middle East respiratory syndrome coronavirus (MERS-CoV) :

    Source Description:S2 subunit of spike (S) protein of MERS-CoV
    Against Virus:

    The Middle East respiratory syndrome coronavirus (MERS-CoV)

    Inhibition Value Type:EC50
    Inhibitory Effect:16.3 ± 1.1
    Inhibitory Unit:µM
    Target Domain Name:
    Assay:Cell–cell fusion assay
    Assay Description:MERS-CoV S protein-mediated cell–cell fusion was assessed, as described previously.(PMID: 25331705, 25451066) In brief, 293T cells expressing MERS-CoV S protein and enhanced GFP (EGFP) were used as the effector cells (293T/MERS/EGFP), and Huh-7 cells expressing the MERS-CoV receptor DPP4 were used as the target cells. 293T/EGFP cells, which express only EGFP, were used as negative control cells. Huh-7 cells were plated in a 96-well plate (5 × 10^4 per well) and cultured at 37 °C for 5 h. Inhibitors at the indicated concentrations were then added, followed by the addition of 293T/MERS/EGFP cells or 293T/EGFP cells (1 × 10^4 per well). After coculture at 37 °C for 4 h, the 293T/MERS/EGFP cells and 293T/EGFP cells fused or unfused with Huh-7 cells were counted under an inverted fluorescence microscope (Nikon Eclipse Ti–S).
    Anti-CoV activity in vivo:
    Reference:29442512
    Comment:
    3D structure:

    Structure Experiment Verified:
    Similar Peptides:ACoVP100284   ACoVP100295   ACoVP100283   ACoVP100294   ACoVP100296