General information
    ACovPid:ACoVP100257
    Trivial Name:GST‐removed HR2
    Amino Acids Sequence:DVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYI
    Length:48
    C-Terminal Modification:None
    N-Terminal Modification:None
    Chemical Modification:None
    Peptide Source:

    Severe acute respiratory syndrome coronavirus (SARS-CoV) : 694009

    Source Description:From the heptad repeat region 2 of spike protein of SARS-CoV
    Against Virus:

    Severe acute respiratory syndrome coronavirus (SARS-CoV) : 694009

    Inhibition Value Type:EC50
    Inhibitory Effect:2.15
    Inhibitory Unit:µM
    Target Domain Name:HR2 domain of SARS-CoV
    Assay:Pseudotyped virus infection inhibition assay
    Assay Description:To produce HIV‐luc/SARS pseudotyped virus, 10 µg of HIV‐1 luciferase reporter vector pNL4.3.Luc.E‐R‐luc (HIV‐luc) and 10 µg of codon‐optimized SARS‐CoV S protein expression plasmid (pcTSh) were co‐transfected into 293 T cells by calcium phosphate coprecipitation. The construction of the plasmids have been performeddescribed previously(PMID: 7531918, 15358126). The pseudotyped virus was harvested after 48 h of incubation and purified by ultracentrifugation through a CsCl density gradient at 50,000g for 4 h. The supernatant containing the pseudotyped virus was collected, filtered through a 0.45 µm Millipore‐sized membrane and stored at −80°C until used. To measure luciferase activity, 20‐µl aliquots of the pseudotyped virus were added to 100 µl of Luciferase Assay Reagent (Luciferase Assay Substrate pre‐mixed with Luciferase Assay Buffer, Promega, USA). Luciferase activity was measure 10 s using a Wallac Multilabel 1450 Counter (Perkin‐Elmer, Singapore). GST‐HR2 and His6‐HR1 were mixed in the presence of ADS‐J1, XXT or PBS, followed by immobilization onto glutathione resin. The resin was then washed with PBS and eluted with 10 mM reduced glutathione. The fractions were separated by SDS–PAGE and analyzed by Western blot using anti‐His tag antibodies. When incubated with PBS only, majority of His6‐HR1 was detected in the elution fractions (Fig. (Fig.6A),6A), showing that His6‐HR1 is bound to GST‐HR2. In the presence of ADS‐J1, His6‐HR1 was only found in the flow‐through but not in the elution fractions (Fig. (Fig.6A),6A), whereas XTT had no effect on the interactions and His6‐HR1 was found only in the elution fractions (Fig. ​(Fig.6B).6B). It indicated that ADS‐J1 interfered with the interactions between His6‐HR1 and GST‐HR2.
    Anti-CoV activity in vivo:
    Reference:18442051
    Comment:
    3D structure:

    StructureACoVP100257

    Structure Experiment Verified:NO
    Similar Peptides:ACoVP100125   ACoVP100119   ACoVP100118   ACoVP100121   ACoVP100120

    Target Domain information
    Target Domain Full Name:Heptad repeat 2 (HR2) domain of Severe acute respiratory syndrome coronavirus (SARS-CoV) spike glycoprotein
    Target Type:glycoprotein
    UniprotID [Sequence]:

    P59594 [1145-1184]

    Target Synonyms:Alternative name(s) for spike glycoprotein: E2 Peplomer protein S glycoprotein
    Target Source:

    Severe acute respiratory syndrome coronavirus (SARS-CoV) : 694009

    Target Structure:1WNC, 1WYY, 1ZV7, 1ZV8, 1ZVB, 2AJF, 2BEQ, 2BEZ, 2DD8, 2FXP, 2GHV, 2GHW, 2RUM, 2RUN, 2RUO, 3BGF, 3D0G, 3D0H, 3D0I, 3SCI, 3SCJ, 3SCK, 3SCL, 5WRG, 5X4S, 5X58, 5X5B, 5XJK, 5XLR, 5ZVM, 6ACC, 6ACD, 6ACG, 6ACJ, 6ACK, 6CRV, 6CRW, 6CRX, 6CRZ, 6CS0, 6CS1, 6CS2, 6M3W, 6NB6, 6NB7, 6VW1, 6WAQ, 7JN5