| General information | |
| ACovPid: | ACoVP100152 |
| Trivial Name: | P3 |
| Amino Acids Sequence: | ISGINASVVNIQKEIDRLNEVAKNLNESLIDLQEL |
| Length: | 35 |
| C-Terminal Modification: | None |
| N-Terminal Modification: | None |
| Chemical Modification: | None |
| Peptide Source: | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) : 2697049 |
| Source Description: | |
| Against Virus: | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) : 2697049 |
| Inhibition Value Type: | EC50 |
| Inhibitory Effect: | 0.72 |
| Inhibitory Unit: | µM |
| Target Domain Name: | |
| Assay: | Cell-cell fusion |
| Assay Description: | Reference:(PMID:24473083):MERS-CoV S protein-mediated cell–cell fusion was assessed using a method similar to that fordetermining HIV-1 Env-mediated cell–cell fusion. Briefly, 293T cells were transfected with the plasmid pAAV-IRES-EGFP encoding the EGFP (293T/EGFP)or pAAV-IRES-MERS-EGFP encoding the MERS-CoV S protein and EGFP(293T/MERS/EGFP) and cultured in DMEM containing 10% FBS at 37°C for 48 h.Huh-7 cells (5*10^4) expressing the MERS-CoV receptor DPP4 were incubated in 96-well plates at 37°C for 5 h, followed by the addition of 1 104293T/EGFPor 293T/MERS/EGFP cells, respectively, in the absence or presence of the testpeptides at graded indicated concentrations. After co-culture at 37°C for 4 h, the293T/MERS/EGFP cells (293T/EGFP cells were used as the negative control) fusedor unfused with Huh-7 cells were counted under an inverted fluorescencemicroscope (Nikon Eclipse Ti-S). The fused cell was much larger by at least two fold than the unfused cell, and the intensity of fluorescence in the fused cell was weakerthan that of the unfused cell as a result of the diffusion of EGFP from one cell totwo or more cells (see Fig. 5a). The percent inhibition of cell–cell fusion wascalculated using the following formula: (1(EN)/(PN))100. ‘E’ representsthe % cell–cell fusion in the experimental group. ‘P’ represents the % cell–cellfusion in the positive control group, to which no inhibitor was added. ‘N’isthe % cell–cell fusion in negative control group, in which 293T/MERS/EGFP cellswere replaced by 293T/EGFP cells. The concentration for 50% inhibition(IC50) was calculated using the CalcuSyn software kindly provided by Dr T.C. Chou60.After further co-culture at 37°C for 48 h, syncytium formation between 293T / MERS / EGFP and Huh-7 could be observed under an inverted microscopewith or without fluorescence (Fig. 5b), and the syncytia were photographedfor the record. |
| Anti-CoV activity in vivo: | |
| Reference: | 32482145 |
| Comment: | |
| 3D structure: | |
| Structure Experiment Verified: | NO |
| Similar Peptides: | ACoVP100242 ACoVP100125 ACoVP100120 ACoVP100119 ACoVP100126 |