| General information | |
| ACovPid: | ACoVP100143 |
| Trivial Name: | SP-4 |
| Amino Acids Sequence: | GFLYVYKGYQPI |
| Length: | 12 |
| C-Terminal Modification: | None |
| N-Terminal Modification: | None |
| Chemical Modification: | None |
| Peptide Source: | Severe acute respiratory syndrome coronavirus (SARS-CoV) : 694009 |
| Source Description: | The peptides of 12 residuesfrom SARS-CoV S protein were synthesized by solid-phasemethod (CytoMol Corp., Union City, CA, USA). The purity(>97%) and composition of peptides were assessed by high-pressure liquid chromatography and electrospray mass spec-tro |
| Against Virus: | Severe acute respiratory syndrome coronavirus (SARS-CoV) : 694009 |
| Inhibition Value Type: | IC50 |
| Inhibitory Effect: | 0.0043±0.00218 |
| Inhibitory Unit: | µM |
| Target Domain Name: | |
| Assay: | Biotinylated enzyme-linked immunosorbent assay(ELISA) |
| Assay Description: | Microtiter plates (MaxiSorp Nunc-ImmumTM plates, Nunc,Denmark) were coated at 4°C overnight with 50ul of spike, ACE2, or bovineserum albumin (BSA) (Sigma, St. Louis, MO, USA), which was diluted in 0.05 M carbonate buffer (16 mM Na2CO2, 34m MNaHCO3, pH 9.6). The wells were rinsed with 200ul wash-ing buffer (0.5% Tween 20 in phosphate-buffered saline (PBS)(137 mM NaCl, 1.4 mM KH2PO4, 4.3 mM Na2HPO4, 2.7 mMKCl, pH 7.2)), and blocked with 200ul blocking buffer (5%BSA in washing buffer) by incubating at 37◦C for 30 min.The absorbed protein in each well was challenged with 50uldiluted biotinylated protein and incubated at 37◦C for 1 h. Afterthree washes with washing buffer, 50ul diluted peroxidase-conjugated avidin was added to each well and incubated at 37◦Cfor 1 h. Following three washes, 50ul chromogenic substrate,2,2′-azinobis(3-ethylbenzthiazoline-sulfonic acid) (Sigma, St.Louis, MO, USA), was added to each well and incubated at37◦C for 15 min. The absorbance was read at 405 nm in an ELISA plate reader (Anthos Labtec Instruments, Austria). For the competition assay, biotinylated S protein (1 nmol) wasmixed with 10 nmol peptide and incubated at 37◦C with shaking.After a 2 h-incubation, the mixture was added to wells, whichwere coated with ACE2, and incubated at 37◦C for 1 h. Follow-ing three washes, peroxidase-conjugated avidin and chromaticsubstrate were sequentially added. The absorbance was read at405 nm in an ELISA plate reader. The inhibition was calculatedby [1−(OD value of mixture containing peptide and spike/ODvalue of mixture containing spike only)]×100. |
| Anti-CoV activity in vivo: | |
| Reference: | 16337697 |
| Comment: | |
| 3D structure: | |
| Structure Experiment Verified: | NO |
| Similar Peptides: | ACoVP100510 |